Histone Tail Modifications of H3 and H4 during the Physarum polycephalum Cell Cycle
نویسنده
چکیده
Problem statement: Histone modifications are required for chromatin activities and are believed to form an epigenetic code. However, the inheritance of the histone post-translational modifications remains unclear. Approach and Results: The aim of this study was to evaluate the histone modifications throughout the cell cycle and to determine whether the histone marks are transferred to daughter cells. Using the naturally synchronous model system Physarum polycephalum and Western blotting analyses, we showed that the histone modifications are not exclusive of the cell cycle stage. Importantly, we also demonstrated that during mitosis, a substantial fraction of the acetylation and the methylation marks of the histone H3 and H4 is removed. Conclusion: The results illustrate that a part of the histone modifications are displaced during mitosis and thus failed to exhibit an inheritance to daughter cells as proposed for the epigenetic marks.
منابع مشابه
Histone synthesis during the cell cycle of Physarum polycephalum. Synthesis of different histone species is not under a common regulatory control.
The synthesis of histones and nonhistone nuclear proteins was studied during the naturally synchronous cell cycle of Physarum polycephalum. Contrary to the commonly accepted idea of a tight coupling of histone biosynthesis and DNA replication during the somatic cell cycle we found that 40% of total histone synthesis takes place in the G2 period in the complete absence of DNA synthesis. The core...
متن کاملH4 replication-dependent diacetylation and Hat1 promote S-phase chromatin assembly in vivo
While specific posttranslational modification patterns within the H3 and H4 tail domains are associated with the S-phase, their actual functions in replication-dependent chromatin assembly have not yet been defined. Here we used incorporation of trace amounts of recombinant proteins into naturally synchronous macroplasmodia of Physarum polycephalum to examine the function of H3 and H4 tail doma...
متن کاملDNA topoisomerase II sites in the histone H4 gene during the highly synchronous cell cycle of Physarum polycephalum.
The nearly perfect synchrony of nuclear division in a plasmodium of Physarum polycephalum provides a powerful system to analyze topoisomerase II cleavage sites in the course of the cell cycle. The histone H4 locus, whose schedule of replication and transcription is precisely known, was chosen for this analysis. Drug-induced topoisomerase II sites are clustered downstream of the histone H4 gene ...
متن کاملBiochemical and morphological characterization of the nuclear matrix during the synchronous cell cycle of Physarum polycephalum.
We have investigated biochemical and ultrastructural aspects of the nuclear matrix during the naturally synchronous cell cycle of Physarum polycephalum. The morphology of the in situ nuclear matrix exhibited significant cell cycle changes as revealed by electron microscopic examination, especially during the progression of nuclei through mitosis and S-phase. In mitosis the interchromatin matrix...
متن کاملEarly activated replication origins within the cell cycle-regulated histone H4 genes in Physarum.
It was previously shown that the two members of the cell cycle-regulated histone H4 gene family, H4-1 and H4-2, are replicated at the onset of S phase in the naturally synchronous plasmodium of Physarum polycephalum, suggesting that they are flanked by replication origins. It was further shown that a DNA fragment upstream of the H4-1 gene is able to confer autonomous replication of a plasmid in...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2014